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为筛选出橡胶树白粉菌Oidium heveae Steimnann新的寄主载体,通过接种野生型拟南芥Col-0、突变体Sr1-4D及eds1,观察了橡胶树白粉菌侵染不同拟南芥突变体的过程,PCR扩增测序法验证相关致病基因,构建橡胶树白粉菌-拟南芥互作体系。结果表明,在Col-0、Sr1-4D叶片上面,橡胶树白粉菌产生部分菌丝后停止生长,不能形成典型的橡胶树白粉病症状;但能成功侵染eds1叶片,在叶片的正、背面有银白色辐射状菌丝,后期在病斑上出现一层粉层,表现橡胶树白粉病的典型症状。组织染色和显微观察结果显示,在突变体eds1叶片上橡胶树白粉菌完成了侵染过程。PCR扩增测序结果表明接种后突变体eds1叶片上及组织中病菌均为橡胶树白粉菌;使用橡胶树白粉菌3个致病相关基因作为靶标,验证了eds1和橡胶树上白粉菌基因组中的3个致病相关基因相似度均达到99%~100%。表明橡胶树白粉菌可侵染拟南芥突变体eds1。
In order to screen a new host vector of Oidium heveae Steimnann, we observed the infection of different Arabidopsis mutants by inoculating wild-type Arabidopsis thaliana Col-0, the mutant Sr1-4D and eds1, and the PCR Amplification and sequencing to verify the related genes and construct the rubber tree powdery mildew - Arabidopsis interaction system. The results showed that the powdery mildew of P. gmelinii ceased to grow on the Col-0 and Sr1-4D leaves and could not form the typical symptom of rubber tree powdery mildew. However, the leaves of eds1 could be successfully infected with silver on the front and back of the leaves White radial mycelium, the latter appeared in the lesion a layer of powder layer, showing the typical symptoms of rubber tree powdery mildew. Tissue staining and microscopic observations revealed that P. gmelinii completes the infection process on mutant eds1 leaves. The result of PCR amplification indicated that the pathogen of eds1 in inoculated mutant was rubber tree powdery mildew; the three pathogenicity-related genes of Rhizoctonia solani were used as target to verify that eds1 and 3 of the genome of powdery mildew of rubber tree Disease-related gene similarity reached 99% to 100%. It showed that rubber tree powdery mildew could infect Arabidopsis mutant eds1.