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目的 探索一种简捷的检测血吸虫循环抗原的方法。方法 应用抗日本血吸虫重组谷胱甘肽 - S-转移酶 (r Sjc2 6 GST)的多克隆抗体标记胶体金 ,建立检测日本血吸虫病患者血清循环抗原的双抗体夹心斑点免疫金渗滤法 (S- DIGFA) ,并以 S- EL ISA作对比。结果 用 S- DIGFA和 S- EL ISA平行检测 95例慢性血吸虫病患者 ,阳性检出率分别为 81.0 5 %和 82 .11% ;正常人血清 180份 ,两者的假阳性率分别为 6 .6 7%和 7.78%。结论 经统计分析证明 S- DIGFA的敏感性和特异性与 S-EL ISA相近 ,且方法更简便快速 ,适合于基层应用。并表明 r Sjc2 6 GST抗原具有诊断血吸虫病的应用价值。
Objective To explore a simple method for detecting circulating antigens of schistosoma japonicum. Methods The polyclonal antibody against Schistosoma japonicum recombinant human glutathione S-transferase (r Sjc2 6 GST) was used to label colloidal gold to establish a sandwich ELISA for detection of circulating antigens in patients with schistosomiasis japonica - DIGFA) and compare with S-EL ISA. Results The 95 cases of chronic schistosomiasis were detected in parallel with S-DIGFA and S-ELISA. The positive detection rates were 81.0 5% and 82.11% respectively. The serum levels of 180 normal individuals were 6 and 6 respectively. 6 7% and 7.78%. Conclusion The statistical analysis proves that the sensitivity and specificity of S-DIGFA are similar to that of S-EL ISA, and the method is simpler and faster, which is suitable for grassroots applications. And showed r Sjc2 6 GST antigen has diagnostic value of schistosomiasis.