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腺相关病毒(adeno-associated virus,AAV)是一类单链线状DNA缺陷性病毒,属细小病毒科,是基因转移最为理想的载体之一。AAV介导肿瘤相关基因感染树突状细胞(dendritic cell,DC)诱导细胞毒性T淋巴细胞(cytotoxic T lymphocyte,CTL),可以内源性持续表达肿瘤相关抗原,并由MHCⅠ类途径得到充分提呈、放大免疫激发CTL的能力,但不影响DC的成熟度。AAV介导抗肿瘤血管生成基因,有抑制肿瘤生长的作用,并且克服了单克隆抗体生产过程中提纯的困难;AAV介导免疫相关基因具有直接抑制肿瘤细胞增殖或免疫调节的作用;AAV介导抑癌相关基因可以诱导肿瘤细胞凋亡;AAV介导自杀基因可以抑制肿瘤的生长。
Adeno-associated virus (AAV) is a type of single-stranded linear DNA-deficient virus, which belongs to the genus Parvoviridae and is one of the most ideal vectors for gene transfer. AAV-mediated dendritic cell (DC) -induced cytotoxic T lymphocyte (CTL) -enhanced tumor-associated gene expression of tumor-associated antigens in endogenous and fully presented by MHC class I , Amplify the ability of immune-stimulated CTL, but do not affect the maturity of DC. AAV mediates antitumor angiogenesis genes and has the effect of inhibiting tumor growth, and overcomes the difficulties of purification in the production of monoclonal antibodies. AAV-mediated immune-related genes have the effect of directly inhibiting tumor cell proliferation or immune regulation; AAV-mediated Tumor-suppressor genes can induce tumor cell apoptosis; AAV-mediated suicide gene can inhibit tumor growth.