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目的 增加评价指标,建立科学、合理的百贝益肺胶囊含量测定方法.方法 C18小柱纯化样品,优化色谱条件为:Vp-ODS色谱柱(150 mm×4.6 mm,5μm);柱温:20℃;变动流速;流动相:乙腈(A)-水(B),梯度洗脱;进样量:10 μL;检测波长:203 nm.结果 所测成分三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1分别在40.24～241.44(r=0.999 6),91.50～549.00(r=0.999 9),35.72～241.32(r=0.999 8)μg·mL-1内线性关系良好;加样回收率为94.15％～99.81％,RSD为1.12％～1.94％.结论 该方法准确,重复性良好,可为修订标准提供依据.“,”OBJECTIVE To augment evaluation index,and establish a scientific and reasonable method for the content determination of Baibei Yifei capsule.METHODS Samples were purified by C18 column,optimized chromatographic conditions:Vp-ODS column (150 mm×4.6 mm,5 μrn);column temperature:20 ℃;fluctuan flow rate;the mobile phase acetonitrile (A)-water (B),gradient elution.The injection volume was 10 μL.The detection wavelength was 203 nm.RESULTS The contents of notoginsenoside R1,ginsenoside Rg1,ginsenoside Rb1 were linear in 40.24-241.44(r=0.999 6),91.50-549.00 (r=0.999 9),35.72-241.32(r=0.999 8)μg·mL-1,respectively.The recovery rate was 94.15％-99.81％,RSD was 1.12％-1.94％.CONCLUSION The method is accurate and reproducible,which can provide the basis for the revision of the standard.