IL-6依赖性MH60.CSF_2细胞株用於测定淋巴细胞培养上清中IL-6生物活性具有灵敏度高、方便易行等优点,但该株细胞在传代培养中易逐渐丧失对IL-6的依赖性。我们将此株细胞亚克隆后,动态观察各代细胞对IL-6依赖性的变化,确定其用於IL-6测定的最好代数、细胞用量和培养时间。现报告如下。1 材料和方法 材料:MH60.CSF_2细胞株由山东省医科院肿瘤生物治疗研究中心田志刚教授惠赠。小鼠rIL-6标准品购自德国Bochringer公司。 IL-6-dependent MH60.CSF_2 cell line for the determination of IL-6 biological activity in lymphocyte culture supernatant has the advantages of high sensitivity, ease and ease, but the cells in the passage of gradual loss of IL-6 Dependency. After subcloning this strain of cells, we dynamically observed the changes of IL-6 in each passage, and determined the best algebra, the amount of cells and the culture time for determination of IL-6. The report is as follows. 1 Materials and Methods Materials: MH60.CSF_2 cell line by the Shandong Provincial Institute of Biomedical Tumor Research Center Professor Tian Zhigang benefit gift. Mouse rIL-6 standard was purchased from the German company Bochringer.