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目的了解卵巢恶性肿瘤患者外周血自然杀伤细胞（ＮＫ细胞）活性及Ｔ淋巴细胞亚群的改变，并探讨其与肿瘤的组织学类型、细胞分化程度及临床分期的关系。方法采用放射性同位素掺入法及ＯＫＴ单克隆抗体，分别检测卵巢恶性肿瘤患者外周血ＮＫ细胞活性及Ｔ淋巴细胞亚群，并采用妇科良性肿瘤患者作对照。结果卵巢恶性肿瘤患者外周血ＮＫ细胞活性较妇科良性肿瘤患者显著降低（Ｐ＜００１），ＯＫＴ４细胞显著减小（Ｐ＜００５），而ＯＫＴ８细胞显著增多（Ｐ＜００５），ＯＫＴ４／ＯＫＴ８比值显著减少（Ｐ＜００５）。ＮＫ细胞活性及Ｔ淋巴细胞亚群的改变与肿瘤的组织学类型、肿瘤细胞分化程度均无关（Ｐ＞００５），而与临床分期有关（Ｐ＜００５），临床分期越晚，其ＮＫ细胞活性越低，ＯＫＴ４细胞越少，ＯＫＴ８细胞越多，ＯＫＴ４／ＯＫＴ８比值越小。结论卵巢恶性肿瘤患者免疫功能是低下的，且临床分期越晚，其免疫功能越低。提示患者的免疫功能与自身体内肿瘤负荷有关。
Objective To investigate the changes of natural killer (NK) cells activity and T lymphocyte subsets in peripheral blood of patients with ovarian cancer, and to explore its relationship with histological types, cell differentiation and clinical stage. Methods The radioactive isotope incorporation method and OKT monoclonal antibody were used to detect the activity of peripheral blood NK cells and T lymphocyte subsets in patients with ovarian cancer. The patients were treated with benign gynecologic tumors. Results The activity of NK cells in peripheral blood of patients with ovarian cancer was significantly lower than that of patients with benign tumors (P <001), OKT4 cells (P <005) and OKT8 cells (P <005) , OKT4 / OKT8 ratio was significantly reduced (P <0 05). The changes of NK cell activity and T lymphocyte subsets were not related with histological types and tumor cell differentiation (P> 005), but correlated with clinical stage (P <005) The lower NK cell activity, OKT4 cells less OKT8 cells, OKT4 / OKT8 ratio smaller. Conclusion The immune function of patients with ovarian cancer is low, and the later the clinical stage, the lower the immune function. Tip of the patient’s immune function and their own tumor load in vivo.