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In order to reduce the cost of Radio Frequency (RF) chains in the spatial multiplexing systems with Vertical-Bell Labs Layered Space-Time (V-BLAST) nonlinear receiver, a novel transmit antenna selection criterion is proposed with the motivation of minimizing the Vector Symbol Error Rate (VSER). In the proposed scheme, both the number of substreams and the mapping of substreams to antennas are dynamically adjusted based on the knowledge of channel. Simulation results illustrate that the proposed two-step selection criterion outperforms the existing eigenmode based selection criterion by 0.3dB at a VSER of 10-3.