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本研究用SJA/9小鼠来评价IgE抗体在虫卵肉芽肿形成中的重要关系。作者选用选择性IgE缺乏的SJA/9小鼠与SJL/J和C57BL/6小鼠作对照。三株小鼠均为12～14周龄、雄性,经腹腔内注射30条日本血吸虫尾蚴,感染后8周在麻醉状态下经下腔静脉放血收集血清,每株小鼠的血清分离后混合。按照Watanabe和Ovary法在Wistar大鼠背部皮肤上作被动皮肤过敏反应测定抗SEA的IgE抗体滴度。小鼠肝组织,经缓冲福尔马林液固定,乙醇脱水,石蜡包埋,制成6μm厚切片,用苏木素伊红染色,按Colley法镜下测量肉芽肿平均直径,并记录虫负荷。
In this study, SJA / 9 mice were used to evaluate the important role of IgE antibodies in granuloma formation. The authors selected SJA / 9 mice with selective IgE deficiency as controls against SJL / J and C57BL / 6 mice. All three mice were 12-14 weeks old. Thirty adult cercariae of Schistosoma japonicum were injected intraperitoneally. Serum was collected from inferior vena cava after anesthesia eight weeks after infection. The serum of each mouse was separated and mixed. Anti-SEA IgE antibody titers were determined according to the Watanabe and Ovary methods on passive cutaneous anaphylaxis in Wistar rat back skin. Mouse liver tissues were fixed in buffered formalin, dehydrated in ethanol and embedded in paraffin to make a 6 μm thick section. The sections were stained with hematoxylin and eosin. The average diameter of granuloma was measured by Colley method and the burden of worms was recorded.