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新生动物注射地塞米松诱发肺气肿模型已广泛用于肺泡发育及相关疾病研究.为探讨地塞米松在肺泡化过程中发挥作用的机制,新生B6小鼠连续腹腔注射地塞米松5-14 d.HE染色进行组织形态学观察,以原位杂交、免疫组化等方法检测肺上皮细胞、血管内皮细胞及弹性蛋白纤维等在肺泡中的分布,以real-time PCR测定PDGF-A、VEGF等肺泡发育相关基因在模型肺组织中的表达.结果显示地塞米松抑制小鼠肺泡化过程,导致肺泡上皮细胞及弹性蛋白纤维分布异常,毛细血管网络发育不良,PDGF-A、VEGF等基因表达水平显著降低.表明地塞米松可能作用于多种肺组织细胞及蛋白因子,阻遏肺泡发生发育.
Neonatal injection of dexamethasone-induced emphysema model has been widely used in alveolar development and related diseases.In order to explore the mechanism of dexamethasone in alveolar process, neonatal B6 mice were injected intraperitoneally dexamethasone 5-14 d.Hematoxylin and eosin staining for histomorphological observation. The distribution of lung epithelial cells, vascular endothelial cells and elastin fibers in the alveoli were detected by in situ hybridization and immunohistochemistry. Real-time PCR was used to detect the expression of PDGF-A, VEGF Etc. The results showed that dexamethasone can inhibit the alveolar process of mice and lead to the abnormal distribution of alveolar epithelial cells and elastin fibers, the poorly developed capillary network, the expression of PDGF-A, VEGF and other genes Level significantly lower, indicating that dexamethasone may act on a variety of lung tissue cells and protein factors, to suppress alveolar development.