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将黄曲霉毒素Ｂ１肟（ＡＦＢ１Ｏ）与牛血清白蛋白（ＢＳＡ）的连接物，通过多点、多次免疫法注射免疫兔子。分析了抗体的产生进程、效价以及特异性。注射抗原后的第６０天开始有较明显抗体产生，第１２０天达到高峰，维持１５天左右后开始下降；抗体的ＥＬＩＳＡ效价高达１：３００００；和黄曲霉毒素Ｂ１（ＡＦＢ１）的结构类似物的竞争ＥＬＩＳＡ表明，抗体有很好的特异性。运用该抗体，以ＥＬＩＳＡ分析检测了几种农产品及饲料中污染ＡＦＢ１的含量，并和薄层层析法的分析结构进行了比较，结果表明当ＡＦＢ１的含量大于等于５ｎｇ／ｍｌ时，两者间有很好的相关性。
The aflatoxin B1 oxime (AFB1O) and bovine serum albumin (BSA) conjugates were injected into rabbits by multipoint immunization. Antibody production process, potency and specificity were analyzed. The first 60 days after the injection of antigens began to have more obvious antibody production, reaching the peak on the 120th day and descending after 15 days; the ELISA titer of the antibody was as high as 1: 30000; and structural analogs of aflatoxin B1 (AFB1) The competition ELISA showed that the antibodies have good specificity. Using this antibody, the content of AFB1 in agricultural products and feedstuffs was detected by ELISA and compared with that of TLC. The results showed that when the content of AFB1 was greater than or equal to 5ng / ml, There is a good correlation.