ｐ５３基因被认为是和多种恶性肿瘤相关的基因，其突变型被认为是癌基因。 ｐ５３基因 ２４９位密码子突变最常见，该突变广泛存在于肝癌、乳腺癌、肺癌、肠癌中。在肺癌的诊断中，纤支镜活检有时钳夹不到组织或组织太小无法确诊。为了提高肺癌的诊断率，我们用ＰＣＲ－ＲＦＬ? The p53 gene is considered to be a gene associated with a variety of malignancies, and the mutant is considered to be an oncogene. Mutations at the 249 codon of the p53 gene are the most common, and this mutation is widely found in liver cancer, breast cancer, lung cancer, and intestine cancer. In the diagnosis of lung cancer, bronchoscopic biopsy sometimes fails to clamp the tissue or the tissue is too small to be diagnosed. In order to improve the diagnostic rate of lung cancer, we use PCR-RFL?