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目的:明确孤儿核受体-雌激素受体相关受体(Estrogen receptor-related receptors,ERRs)α-1亚型在卵巢癌组织中的表达情况及其临床意义。方法:采用实时荧光定量RT-PCR和免疫组化方法检测人ERRα-1在45例卵巢癌组织标本和15例正常卵巢组织中的mRNA和蛋白表达情况;采用ELISA方法分析各样本的血清CA125水平;随访指标为整体生存时间和无瘤生存时间。结果:免疫组化分析表明ERRα-1在卵巢癌组织中的表达率显著高于正常组织(P<0·05)。定量PCR分析表明卵巢癌组织中ERRα-1的mRNA平均表达丰度显著高于正常卵巢组织(P<0·05)。卵巢癌患者血清CA125水平分析显示ERRα-1阳性组CA125明显升高(P<0·05)。随访结果显示ERRα-1阳性的卵巢癌患者的整体生存时间比ERRα-1阴性患者明显减少(P<0·05),但两组无瘤生存时间并无差异(P>0·05)。结论:ERRα-1可能在卵巢癌的发生发展中起重要作用,ERRα-1表达增加与卵巢癌的预后不良有关。
Objective: To clarify the expression of orphan nuclear receptor-related receptors (ERRs) α-1 in ovarian cancer and its clinical significance. Methods: The mRNA and protein expressions of ERRα-1 in 45 specimens of ovarian cancer and 15 specimens of normal ovarian tissue were detected by real-time fluorescence quantitative RT-PCR and immunohistochemistry. The levels of serum CA125 in each sample were analyzed by ELISA ; Follow-up indicators for the overall survival time and tumor-free survival time. Results: Immunohistochemical analysis showed that the expression of ERRα-1 in ovarian cancer was significantly higher than that in normal tissues (P <0.05). Quantitative PCR analysis showed that the average expression level of ERRα-1 mRNA in ovarian cancer tissues was significantly higher than that in normal ovarian tissues (P <0.05). Analysis of serum CA125 levels in patients with ovarian cancer showed that CA125 of ERRα-1 positive group was significantly higher (P <0.05). The follow-up results showed that the overall survival time of ERRα-1 positive ovarian cancer patients was significantly lower than that of ERRα-1 negative patients (P <0.05), but no difference was found between the two groups (P> 0.05). Conclusion: ERRα-1 may play an important role in the development of ovarian cancer. The increased expression of ERRα-1 is associated with the poor prognosis of ovarian cancer.