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目的 :研究人参皂苷Rg1和Rh1对树突状细胞 (dendriticcell,DC)功能的影响。方法 :用ELISA法检测人参皂苷Rg1及Rh1对DC的IL - 12p4 0蛋白产生量的影响 ,用RT -PCR检测人参皂苷Rg1及Rh1对DCIL - 12p4 0mRNA表达水平的影响。用中性红吞噬法检测人参皂苷Rg1及Rh1对DC -LPAK对肿瘤细胞的杀伤作用。结果 :ELISA结果表明 ,Rg1和Rh1各剂量组均能显著提高DCIL - 12p4 0蛋白的产量。与对照组相比 ,Rg11mg/L及Rh110 0mg/L可明显增加DCIL - 12p4 0mRNA的转录 ,与其蛋白表达相一致。Rg1及Rh1均能促进DC -LPAK对乳头瘤细胞的杀伤能力 ;在对L92 9杀伤实验中 ,效靶比为 5∶1时 ,Rg1各剂量均能显著促进DC -LPAK的杀伤活性(P <0 0 1,P <0 0 5 ) ,而Rh1仅中剂量能提高DC -LPAK的杀伤活力 (P <0 0 5 )。结论 :Rg1和Rh1通过增强IL - 12p4 0mRNA的表达来增加其蛋白的产量。并且 ,由于IL - 12产量的增加从而增强了DC -LPAK对肿瘤细胞的杀伤能力
Objective: To study the effects of ginsenosides Rg1 and Rh1 on the function of dendritic cells (DCs). METHODS: The effects of ginsenosides Rg1 and Rh1 on the production of DC-12p4 0 were detected by ELISA. The effects of ginsenosides Rg1 and Rh1 on the expression of DCIL-12p4 0 mRNA were detected by RT-PCR. Neutrophic phagocytosis was used to detect the killing effects of ginsenoside Rg1 and Rh1 on DC-LPAK on tumor cells. Results : ELISA results showed that Rg1 and Rh1 doses significantly increased the production of DCIL-12p4 0 protein. Compared with the control group, Rg11mg/L and Rh110 0mg/L significantly increased the transcription of DCIL-12p4 0 mRNA, which was consistent with its protein expression. Both Rg1 and Rh1 could promote the killing of DC-LPAK on papilloma cells. In the L92 9 killing experiment, when the ratio of effect to target was 5:1, each dose of Rg1 significantly promoted the killing activity of DC-LPAK (P < 0 0 1, P <0 0 5 ), while only medium dose of Rh1 increased the killing activity of DC-LPAK (P <0 05). CONCLUSION: Rg1 and Rh1 increase the production of IL-12p4 0 mRNA by increasing its protein production. And, due to the increase of IL-12 production, the killing ability of DC-LPAK on tumor cells is enhanced.