目的探讨检测试剂室温放置时间因素对心肌酶谱五项测定结果的影响。方法首先准备心肌酶谱检测试剂,室温放置0h、2h、6h、24h、48h和96h,然后利用TOSHIBA-120FR型全自动生化分析仪测定心肌梗死42例、心肌炎30例、健康体检28例的心肌酶谱AST、LDH、CK、CK-MB和HBDH五项指标,观察试剂放置时间因素对结果的影响。结果试剂27℃放置0~96h,心肌酶谱AST、LDH、CK、CK-MB和HBDH五项酶活性指标未发现有逐渐增高或降低的现象。结论心肌酶谱5项指标生化检测试剂室温放置4d测定结果基本稳定,试剂室温放置4d以内可用于临床检测。 Objective To investigate the influence of testing reagent temperature at room temperature on the results of five determinations of myocardial enzymes. Methods Myocardial enzymology detection reagent was prepared at room temperature for 0h, 2h, 6h, 24h, 48h and 96h, and then TOSHIBA-120FR automatic biochemical analyzer was used to determine myocardial infarction in 42 cases, myocarditis in 30 cases and healthy subjects in 28 cases Zymogram AST, LDH, CK, CK-MB and HBDH five indicators, the effect of reagent placement time on the results. Results Reagents 27 ℃ placed 0 ~ 96h, myocardial enzymes AST, LDH, CK, CK-MB and HBDH five enzyme activity index did not find a gradual increase or decrease phenomenon. Conclusions The results of cardiac biochemical detection of 5 indexes of biochemical detection reagent for 4 days at room temperature are basically stable. The reagents can be used for clinical examination within 4 days at room temperature.