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目的 研究大鼠肝实质细胞癌细胞 (HTC)的粘弹特性及其与胶原蛋白Ⅳ裱衬人工基底膜的粘附特性 ,分析其在细胞侵袭转移过程中的生物学意义。方法 同步化G1期 ( 69.2 0 %～72 .10 % )和S期 ( 97.18%～ 98.94% )HTC细胞以胸腺嘧啶核苷和秋水仙碱顺序阻断法及胸腺嘧啶核苷双阻断法分别获得 ,以微管吸吮技术对细胞的粘弹性及其与人工基底膜的粘附性进行测定。结果 HTC细胞具有高弹性系数 (K1、K2 )和低粘性系数 ( μ)的特征 ( 186.5 2± 75 .60 )N/m2 、( 2 2 4.42± 114 .5 2 )N /m2 、( 3.12± 2 .2 5 )M·S·m-2 ,G1期细胞尤为明显 ( 2 15 .2 8± 5 0 .2 4)N/m2 、( 181.92± 10 2 .90 )N/m2 、( 2 .87± 1.2 7)M·S·m-2 ,与胶原蛋白Ⅳ裱衬的人工基底膜的粘附力与胶原蛋白Ⅳ的浓度密切相关 ,G1期细胞 ( 2 75 .86± 2 32 .80 )× 10 -10 N具有比S期细胞( 161.16± 12 0 .40 )× 10 -10 N更高的粘附力和标准差 ,差异有显著性 (P <0 .0 5 )。结论 HTC细胞 ,尤其是其G1期细胞 ,更有可能在血液循环中存活并直接在微循环中滞留粘附 ,并穿透血管基底膜实现远端转移。
Objective To study the viscoelastic characteristics of rat hepatic parenchymal cancer cell (HTC) cells and its adhesion to collagen IV-grafted artificial basilar membrane, and analyze its biological significance in cell invasion and metastasis. METHODS: Synchronized thymidine and colchicine sequential blockade and thymidine double blockade were performed in synchronized G1 (69.20% to 72.10 %) and S phase (97.18% to 98.94%) HTC cells, respectively. Obtained, the viscoelasticity of the cells and their adhesion to the artificial basement membrane were measured by micropipette suction technique. Results The characteristics of HTC cells were high elastic coefficient (K1, K2) and low viscosity coefficient (μ) (186.5 2± 75 .60) N/m2, (2.24± 114.52) N/m2, (3.12±) 2.25)M·S·m-2, especially in G1 phase cells (2 15 0.28 ± 5 0.24) N/m 2 , (181.92± 10 2 .90) N/m 2 ,( 2 . 87 ± 1.2 7) M·S·m-2 , adhesion to the collagen IV-liner artificial basement membrane is closely related to the concentration of collagen IV, G1 phase cells (2 75.86 ± 2 32.80) X 10 -10 N had higher adhesion and standard deviation than S-phase cells (161.16± 12 0 .40) × 10 -10 N, with a significant difference (P < 0.05). Conclusion HTC cells, especially their G1 phase cells, are more likely to survive in the blood circulation and directly adhere to the microcirculation and penetrate the vascular basement membrane to achieve distal metastasis.