论文部分内容阅读
【目的】探讨清热活血调气方对坏死性小肠结肠炎(NEC)模型新生大鼠回肠组织Toll样受体4(TLR4)mRNA、核因子-κB(NF-κB)mRNA表达及细菌易位的影响。【方法】选用3日龄SD新生大鼠随机分为3组:正常对照组、NEC模型组及治疗组。采用鼠代乳品人工喂养及缺氧冷刺激的方法,复制新生大鼠NEC模型。治疗组采用鼠乳代用品+清热活血调气方(15 g.kg-1.次-1,3次/d)人工喂养,同时予以缺氧冷刺激;正常对照组鼠乳喂养,不进行任何干预。第4天心脏穿刺采血后处死大鼠取近回盲部末端回肠2 cm,采用实时荧光定量PCR法检测回肠组织TLR4 mRNA、NF-κB mRNA表达水平及血标本中细菌16S rRNA进而得出细菌易位发生率。【结果】与模型组比较,治疗组TLR4 mRNA、NF-κB mRNA表达水平显著下降(P<0.01),细菌易位发生率显著降低(P<0.05)。【结论】清热活血调气方可下调NEC模型新生大鼠回肠组织TLR4 mRNA、NF-κB mRNA表达,减少细菌易位的发生。
【Objective】 To investigate the effects of “Qingre Huoxue Tiangong Recipe” on Toll-like Receptor 4 (TLR4) mRNA, NF-κB mRNA expression and bacterial translocation in ileal tissue of neonatal rats with necrotizing enterocolitis (NEC) influences. 【Methods】 Three-day-old SD neonatal rats were randomly divided into three groups: normal control group, NEC model group and treatment group. Neonatal rat model of NEC was reproduced by artificial feeding and hypoxia cold stimulation of rat substitutes. The rats in the treatment group were fed with the milk substitutes and the heat-clearing and blood circulation-regulating herbs (15 g.kg-1. Times-1, 3 times / d) Intervention. On day 4, the rats were sacrificed by cardiac puncture and the ileum was resected for 2 cm. The real-time fluorescent quantitative PCR method was used to detect the expression of TLR4 mRNA and NF-κB mRNA in the ileum and the bacterial 16S rRNA Bit rate. 【Results】 Compared with the model group, the expressions of TLR4 mRNA and NF-κB mRNA in the treatment group were significantly decreased (P <0.01), and the incidence of bacterial translocation was significantly decreased (P <0.05). 【Conclusion】 Qingre Huoxue Tiaogan can down-regulate the expression of TLR4 mRNA and NF-κB mRNA in the ileum of neonatal rats with NEC model and reduce the occurrence of bacterial translocation.