论文部分内容阅读
目的 探讨阿司匹林 (aspirin ,As)在抗H2 O2 损伤内皮细胞过程中对铁蛋白 (ferritin ,Fn)表达的作用。方法 体外培养人血管内皮细胞 ,酶联免疫吸附法 (ELISA)测定As在不同浓度 (0 1～ 3mmol/L)、不同处理时间 (4～ 2 4h)对细胞Fn表达的影响 ,以及消炎痛和水杨酸钠对Fn表达的作用 ;并观察经As预处理后的细胞加入H2 O2 (0 5mmol/L)后乳酸脱氢酶 (lactatedehydrogenase ,LDH)释放率、丙二醛 (malondialdehyde,MDA)和细胞存活率的改变。用单因素方差分析对上述指标进行统计学处理。结果 小剂量As (0 1mmol/L)即可明显诱导内皮细胞Fn表达 (5 8ng/ 10 6细胞数± 0 3ng/ 10 6细胞数 ) ,与正常对照组比较P <0 0 5 ;且As与Fn之间表现出剂量和时间依赖关系 ,0 5mmol/L、1mmol/L、2mmol/L、3mmol/L组Fn浓度分别为 (6 4± 0 4)ng/ 10 6细胞数、(7 0± 0 7)ng/ 10 6细胞数、(7 4±0 4)ng/ 10 6细胞数和 (7 7± 0 5 )ng/ 10 6细胞数 ;4h组Fn尚未明显增加 (5 8ng/ 10 6细胞数± 1 0ng/10 6细胞数 ,P >0 0 5 ) ,但 8h组Fn浓度 (6 5± 1 0 )ng/ 10 6细胞数与正常对照组比较P <0 0 5 ,2 4h组Fn浓度达最大值 (7 8± 0 8)ng/ 10 6细胞数。As诱导Fn表达后能明显增强细胞抗氧化的能力 ,0 1mmol/L的As可减
Objective To investigate the effect of aspirin (As) on the expression of ferritin (Fn) in endothelial cells induced by H2O2 injury. Methods Human vascular endothelial cells were cultured in vitro. The effect of As on the expression of Fn in different concentrations (0 ~ 3mmol / L) and different treatment time (4 ~ 24h) was measured by enzyme linked immunosorbent assay (ELISA) The effect of sodium salicylate on the expression of Fn was observed. The release of lactate dehydrogenase (LDH), malondialdehyde (MDA), and Changes in cell survival. One-way analysis of variance using the above indicators for statistical processing. Results Low dose As (0 1 mmol / L) could obviously induce the expression of Fn in endothelial cells (5 8 ng / 106 cells ± 0.3 ng / 106 cells), compared with the normal control group Fn showed a dose-dependent and time-dependent manner. The Fn concentrations of 0 5 mmol / L, 1 mmol / L, 2 mmol / L and 3 mmol / L groups were (6 4 ± 0 4) (7 4 ± 0 4) ng / 10 6 cells and (7 7 ± 0 5) ng / 10 6 cells, while Fn in 4h group had no significant increase (58ng / 106 The number of cells ± 10ng / 106 cells, P> 0.05), but the number of Fn in 8h group was (65 ± 10) ng / 106 cells compared with the normal control group P <0.05, 24h group Fn concentration reached the maximum (78 ± 0 8) ng / 106 cells. As induced the Fn expression can significantly enhance the cell antioxidant capacity, 0 1mmol / L of As can be reduced