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作者采用三种方法,进行了皮肤表皮细胞培养共120例。并初步应用于临床。表皮细胞接种于胶原者,7～10天呈片,形成2～4层细胞,培养结果稳定。但细胞和胶原紧贴,不能单独分离。培养在3T3细胞滋养层者,于2～3周内形成3～8层复层细胞片,生长稳定。传代最多至第六代。由于3T3细胞是异种细胞,故未用于临床。表皮细胞接种于玻璃瓶进行无基底物培养,1～2天就有新细胞增殖,6～10天已形成整张复层细胞片,最多达15层以上,相似于人体皮肤表皮层结构。生长稳定,能进行多次传代,容易从瓶中分脱,适合于移植。作者将培养的细胞膜片移植于削痂创面及供皮区共20例,23例次。移植细胞膜片能加速创面愈合,移植后存活情况满意。
The authors adopted three methods, a total of 120 cases of skin epidermal cell culture. And initially applied to clinical. Epidermal cells were seeded on collagen, 7 to 10 days was film, forming 2 to 4 layers of cells, the culture results were stable. But cells and collagen close, can not be separated alone. Trophoblast cultured in 3T3 cells, in 2 to 3 weeks to form 3-8 layers of cell sheet, stable growth. Passage up to the sixth generation. Since 3T3 cells are xenogenic cells, they are not used clinically. Epidermal cells seeded in glass bottles for substrate-free culture, 1 to 2 days there is a new cell proliferation, 6 to 10 days has formed a whole sheet of cells, up to 15 layers, similar to the human skin epidermal structure. Stable growth, capable of multiple passages, easy to detach from the bottle, suitable for transplantation. The cultured cells were transplanted in 20 cases of scab wounds and donor sites for 23 cases. Transplantation of cell membrane can accelerate wound healing, survival after transplantation satisfactory.