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从1981年10月至1982年5月,在美国伊利诺斯大学植病系温室中接种并鉴定了中国大豆花叶病毒的标样23个。根据它们在9个抗性不同的鉴别品种上的反应差异,分为10个株系群,即在原来 G_1—G_7的7群基础上增设3个群,它们是 G_8、G_9和G_10,分别以 C_(16),S_6和 C_(28)3个标样为代表。这3群6个毒株的毒力要比美国7群的都强些,一些在美国认为是高抗或免疫的品种,在感染中国毒株后表现为黄色斑或叶脉坏死的症状。用十二烷基磺酸钠(S.D.S)配制的琼脂凝胶平板进行奥氏双扩散血清学检验,大豆花叶病毒与相应的抗血清发生沉淀反应,乳白色沉淀带清晰可见。
From October 1981 to May 1982, 23 samples of China soybean mosaic virus were inoculated and identified in the greenhouse of the Department of Plant Diseases, University of Illinois, USA. According to their differences in response to 9 different resistant cultivars, 10 lines were divided into three groups: G_8, G_9 and G_10 on the basis of the original G_1-G_7 group C_ (16), S_6 and C_ (28) three standard samples as the representative. The virulence of these 6 strains of 6 strains is stronger than that of 7 colonies in the United States. Some species that are considered highly resistant or immunized in the United States exhibit symptoms of yellowish or necrosis of the vein after infection with Chinese strains. Soybean mosaic virus (SDS-PAGE) was used to perform serological tests on the agarose gel plates prepared with sodium dodecyl sulfate (S.D.S). The milky white precipitate was clearly visible.