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目的 探讨一氧化氮(NO)在实验性反流性食管炎发病中的作用。方法 48 只SD大鼠随机分为3 组:A组:采用完全幽门结扎+ 贲门肌切开术;B组:采用半幽门缝扎+ 贲门肌切开术,分别制备反流性食管炎动物模型;C组:假手术对照组。并于模型制备后24小时、48 小时、72 小时观察食管下端粘膜的病理表现,测定食管组织的NO含量。结果 食管炎指数(肉眼和显微镜下),A 组术后24小时以及B组术后24 小时、48 小时、72 小时与C组比较差异均有非常显著意义( P< 0.01 );食管组织NO含量,A 组术后24小时以及B组术后24小时、48小时、72小时与C组比较,差异亦均有显著性意义(P< 0.05, P< 0.01 );不同程度食管炎组的食管组织NO 含量均明显高于非食管炎组(P<0.05,P< 0.01 ),而不同程度食管炎组间的食管组织NO含量差异无显著性意义(P> 0.05 )。结论 NO在实验性反流性食管炎发病机制中对食管粘膜可能发挥损伤和保护的双重作用
Objective To investigate the role of nitric oxide (NO) in the pathogenesis of experimental reflux esophagitis. Methods 48 SD rats were randomly divided into 3 groups: group A: complete pyloric ligation + cardia myotomy; group B: semi-pyloric suture + cardiomyotomy, respectively, to prepare animal models of reflux esophagitis ; C group: sham operation control group. The pathological findings of the lower esophageal mucosa were observed 24 hours, 48 hours and 72 hours after the model was prepared, and the content of NO in esophageal tissue was measured. Results The esophagitis index (under macroscopic and microscopic) showed that there was significant difference between group A and group C at 24 hours after operation and at 24 hours, 48 hours and 72 hours after operation in group B (P <0.01). The content of NO in esophageal tissue (P <0.05, P <0.01). The difference of esophageal tissue between group A and group C was significant at 24 hours after operation and at 24 hours, 48 hours and 72 hours after operation in group B (P <0.05, P <0.01). No significant difference was found in the content of NO in esophageal tissues between different esophagitis groups (P> 0.05). Conclusion NO may play a dual role in the pathogenesis of esophageal mucosa injury and protection in the pathogenesis of experimental reflux esophagitis