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目的 :观察重组人活化素A(rhAcT)对MPP+ 所诱导的PC12细胞损伤的保护作用。方法 :将MMP+ 或活化素A ,L deprenyl加入体外培养的PC12细胞 ,用四甲基偶氮唑盐 (MTT)法检测细胞活力的变化 ;用免疫细胞化学法和RT PCR评价细胞的酪氨酸羟化酶和bcl 2蛋白及mRNA含量的变化 ,用脱氧核苷酸末端转移酶介导的缺口末端标记法 (TUNEL)检测细胞凋亡的变化 ,比较各组的差异。结果 :预先给予rhAcT和L deprenyl的两组细胞活力明显高于MPP+ 损害组 ,酪氨酸羟化酶和bcl 2的蛋白及mRNA表达强于损害组 ,同时两组的凋亡细胞明显减少 ,rhAcT和L deprenyl两组间无显著差异。结论 :rhAcT和L deprenyl通过上调bcl 2的表达 ,抑制凋亡的发生 ,而对MMP+ 所诱导的PC12细胞的损伤具有保护作用。
Objective: To observe the protective effect of recombinant human activin A (rhAcT) on the injury of PC12 cells induced by MPP +. Methods: MMP12 or activin A and L deprenyl were added into PC12 cells cultured in vitro. The cell viability was detected by MTT assay. The cytotoxicity of tyrosine was evaluated by immunocytochemistry and RT PCR Hydroxylase and bcl 2 protein and mRNA levels. The changes of apoptosis were detected by TUNEL. The differences among groups were compared. Results: The cell viability of both rhAcT and L deprenyl groups was significantly higher than that of MPP + lesion group. The protein and mRNA expressions of tyrosine hydroxylase and bcl 2 were stronger than those of the injured group. Meanwhile, the apoptotic cells in both groups were significantly decreased. And L deprenyl no significant difference between the two groups. Conclusion: rhAcT and L deprenyl can inhibit the apoptosis of PC12 cells induced by MMP + by up-regulating the expression of bcl-2.