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本文对慢粒及慢粒急性变患者从P_(53)的DNA、RNA和蛋白质水平上进行了系列研究,对比其不同的P_(53)基因突变率、mRNA表达率、以及P_(53)蛋白的变化,结果指出:P_(53)基因在慢粒急变和加速期患者中的突变频率(25％)远高于慢性期患者(5.56％),而P_(53)的表达率前者(50％)也与后者截然不同(100％),在二例P_(53)蛋白阳性的慢粒慢性期患者中,一例在进入加速期后P_(53)蛋白转呈阴性,也进一步说明抗癌基因P_(53)的突变与慢粒慢性急变有关。
In this paper, a series of studies have been conducted on the DNA, RNA, and protein levels of P_(53) in patients with chronic and chronically acute mutations, comparing their different P_53 gene mutation rates, mRNA expression rates, and P_53 protein. The results indicate that the mutation frequency of the P_53 gene in patients with CRM and accelerated phase (25%) is much higher than that in patients with chronic phase (5.56%), while the expression rate of P_53 is the former (50%). ) It is also distinct from the latter (100%). In the two patients with chronic P53 protein-positive chronic phase, one case showed negative P53 protein after entering the accelerated phase, which further explains the anti-oncogene. Mutations in P_(53) are associated with chronic, chronic, rapid changes.