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本文采用CaCl_2-ACh混合液给小鼠静注形成房颤(扑),对照组(80只)房颤(扑)阳性率达80—100％。实验观察了几种已知抗心律失常药物的对抗作用,奎尼丁的作用最强;异搏定也有一定作用;当使用大于临床应用的剂量时,心得安、苯妥英钠也有对抗作用;利多卡因则基本无效。实验结果大致符合这些药物在临床上的选择性应用。本实验方法具有所需费用低廉、操作方法简单及观察指标明确等优点,可作为筛选治疗房颤(扑)药物的初筛方法。 多年来,治疗室性心律失常的药物已取得很大进展,但治疗房颤和房扑的新药进展不大,仍用毒、付作用较多的洋地黄和奎尼丁。在抗心律失常药物的实验研究中,目前所采用的房颤(扑)实验模型多用较大动物,并且实验步骤较繁琐,如给狗开胸后在心房安放电极刺激或局部用药(乌头碱、乙酰胆碱等);或用药物使狗造成甲状腺机能亢进或低血钾状态,然后给予大剂量乙酰胆碱诱发房颤(扑)。这些方法虽具有许多优点,但不适用于大量药物的筛选。 本文采用CaCl_2-ACh混合液给小鼠静注形成房颤(扑)的简易动物实验模型,并用几种已知抗心律失常药进行了实验观察。
In this paper, a mixture of CaCl_2-ACh to mice was injected to form atrial fibrillation (flutter), the control group (80) atrial fibrillation (flutter) positive rate of 80-100%. Experiments were conducted to observe the antagonistic effects of several known anti-arrhythmic drugs, quinidine’s strongest effect; verapamil also have a role; when the use of more than the clinical dose, propranolol, phenytoin sodium also antagonism; lidoca Because of the basic ineffective. The experimental results are broadly in line with the clinical selectivity of these drugs. The experimental method has the advantages of low cost, simple operation method and clear observation index, which can be used as a screening method for screening drugs for atrial fibrillation (flutter). Over the years, the treatment of ventricular arrhythmia drugs have made great progress, but the treatment of atrial fibrillation and atrial flutter little progress in new drugs, still use drugs to pay more effect of digitalis and quinidine. In the experimental study of anti-arrhythmic drugs, the current experimental model of atrial fibrillation (FL) used more animals, and the experimental steps are more complicated, such as placing the electrodes in the atrium of the dogs after thoracotomy for stimulation or topical application (aconitine , Acetylcholine, etc.); or drugs to cause dogs with hyperthyroidism or hypokalemia, and then given a large dose of acetylcholine-induced atrial fibrillation (flutter). Although these methods have many advantages, they are not suitable for screening large quantities of drugs. In this paper, a simple animal experimental model of atrial fibrillation was established by intravenous injection of CaCl 2 -ACh mixture, and several known anti-arrhythmic drugs were used for experimental observation.