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血清中硫酸盐总量测定的原理是用三氯醋酸沉淀血清中蛋白质后,加浓盐酸水解滤液中的酯键硫酸盐,并与联苯胺形成联苯胺——硫酸盐的沉淀,再以稀盐酸溶解沉淀,释放出联苯胺,此物与三氯化铁和过氧化氢显色、比色测定。此法是以联苯胺的量表示硫总量,但加入的浓盐酸也可与联苯胺结合生成联苯胺一盐酸盐的沉淀,因而联苯胺一硫酸盐的沉淀中混有联苯胺盐酸盐,这样污染的沉淀物溶于稀盐酸后所释放出的联苯胺必然增加,所测值必然升高。另外由于所加浓盐酸的多少不同(滴有大、有小),形
The principle of determination of the total amount of sulfate in serum is to precipitate the protein in the serum with trichloroacetic acid, add the ester bond sulfate in the hydrochloric acid hydrolysis filtrate and form the benzidine-sulfate precipitate with the benzidine, Solubilization precipitation, the release of benzidine, this material with ferric chloride and hydrogen peroxide color, colorimetric determination. This method is based on the amount of benzidine said that the total sulfur, but the addition of concentrated hydrochloric acid can also be combined with the benzidine to generate benzidine monohydrochloride precipitation, which benzidine monosulfate precipitation mixed with benzidine hydrochloride , So contaminated sediment dissolved in dilute hydrochloric acid released benzidine bound to increase, the measured value will inevitably increase. In addition due to the amount of concentrated hydrochloric acid is different (dripping large, small), shaped