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目的建立对氧磷酶（ｐａｒａｏｘｏｎａｓｅ，ＰＯＮ）活性测定方法，调查正常人及肝病患者（肝癌与肝硬化）血清ＰＯＮ活性。方法ＰＯＮ活性测定用分光光度法，同时检验批内差异、批间差异、重复性、线性关系及回收率。结果正常人血清ＰＯＮ活性平均值为１５４．３ｋＵ／Ｌ，参考值范围为８２．１～２２０．６ｋＵ／Ｌ，肝病患者ＰＯＮ活性平均值为８１．１ｋＵ／Ｌ。结论肝病患者血清ＰＯＮ活性较正常人明显下降，其差别有显著性（Ｐ＜０．０１）。
Objective To establish a method for the determination of paraoxonase (PON) activity and to investigate the serum PON activity in normal and liver disease patients (liver cancer and cirrhosis). Methods PON activity was measured spectrophotometrically, while testing intra-assay differences, inter-assay differences, repeatability, linearity and recovery. Results The average serum PON activity of normal people was 154.3 kU / L, the reference range was 82.1 ~ 220.6 kU / L, and the average PON activity of liver disease patients was 81.1 kU / L. Conclusion The serum PON activity of patients with liver disease is significantly lower than that of normal people (P <0.01).