The effects of four microalgae,Chlorella vulgaris,Platymonas helgolandicavar,Isochrysis galbana,and Nitzschia closterium on the grazing and filtering rates of the marine rotifer,Brachionus plicatilis,were evaluated under laboratory conditions.The grazing rates in separate cultures of the four microalga were as follows:C.vulgaris > P.helgolandicavar > I.galbana > N.closterium.However,the filtering rates occurred in the following order:P.helgolandicavar > N.closterium > C.vulgaris > I.galbana.A mixed diets experiment revealed that P.helgolandicavar was the preferred diet of B.plicatilis.In addition,the grazing rate of B.plicatilis increased gradually as the density of the microalgae increased,until concentrations of 2.5×106 cells mL-1 for C.vulgaris and 1.5×106 cells mL-1 for I.galbana were obtained.Furthermore,the filtering rate increased slightly when the density of the microalgae was low,after which it declined as the microalgal density increased.The grazing rates of B.plicatilis were as follows during the different growth phases:stationary phase > exponential phase > lag phase > decline phase.Additionally,the filtering rates during the growth phases were:exponential phase > lag phase > stationary phase > decline phase.The results of this study provide foundational information that can be used to explore the optimal culture conditions for rotifers and to promote the development of aquaculture. The effects of four microalgae, Chlorella vulgaris, Platymonas helgolandicavar, Isochrysis galbana, and Nitzschia closterium on the grazing and filtering rates of the marine rotifer, Brachionus plicatilis, were evaluated under laboratory conditions. The grazing rates in separate cultures of the four microalga were as C.vulgaris> P.helgolandicavar> I.galbana> N.closterium.However, the filtering rate occurred in the following order: P.helgolandicavar> N. closterium> C. vulgaris> I.galbana. A mixed diets laboratory revealed that P.helgolandicavar was the preferred diet of B.plicatilis.In addition, the grazing rate of B.plicatilis increased gradually as the density of the microalgae increased, until concentrations of 2.5x106 cells mL-1 for C. vulgaris and 1.5x 106 cells mL-1 for I. galbana were obtained. Fermentmore, the filtering rate increased slightly slightly when the density of the microalgae was low, after which it declined as the microalgal density increased. The grazing rates of B. plicatilis we re as follows during the different growth phases: stationary phase> exponential phase> lag phase> decline phase. Additionally, the filtering rates during the growth phases were: exponential phase> lag phase> stationary phase> decline phase. The results of this study provide foundational information that can be used to explore the optimal culture conditions for rotifers and to promote the development of aquaculture.