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应用巢式聚合酶链反应（ＰＣＲ法）对１４８例维持性血液透析病人进行了血清丙肝病毒（ＨＣＶ）感染标志物ＨＣｖＲＮＡ检测，同时应用ＥＬＩＳＡ法检测了血清抗ＨＣＶ，结果显示：１４８例血透病人共检出ＨＣＶＲＮＡ２１例，阳性率１４．２％，抗ＨＣＶ１５例，阳性率１０．１％。并对年龄、性别和透析时间各不相同的血透病人所检测出的丙肝病毒标志物ＨＣＶＲＮＡ阳性结果进行分析，结果发现丙肝病毒感染的发生率与血透病人的性别、年龄无关，与血透时间密切相关，提示透析时间越长感染丙肝病毒危险性越大。
HCN was detected by nested polymerase chain reaction (PCR) in 148 patients with maintenance hemodialysis and serum anti-HCV was detected by ELISA. The results showed that 148 cases of hemodialysis A total of 21 patients were detected HCVRNA, the positive rate was 14.2%, anti-HCV15 cases, the positive rate of 10.1%. Hepatitis C virus (HCV) positive HCV RNA detected by hemodialysis patients with different age, sex and dialysis time were analyzed. The results showed that the incidence of HCV infection was not related to the gender and age of hemodialysis patients, Time is closely related, suggesting that the longer dialysis the greater the risk of hepatitis C infection.