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[目的]观察不同剂量慢性锰染毒对大鼠学习记忆能力及其对血浆脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)含量的影响,探讨血浆BDNF含量作为锰致大鼠学习记忆功能损害效应生物标志的可行性。[方法]将40只雄性SD大鼠随机分为对照组及低(5 mg/kg)、中(10 mg/kg)、高(20 mg/kg)剂量染猛组,腹腔注射染毒,每周5 d,连续18周。分别于染毒第6、12、18周,通过Morris水迷宫实验检测大鼠的学习记忆能力。于第18周末次染毒24 h后,采血并分离血浆,用石墨炉原子吸收光谱法和ELISA试剂盒分别检测大鼠血浆锰及BDNF含量。[结果]在第6周的水迷宫实验中,与对照组相比,高剂量组逃避潜伏期延长,穿越平台次数下降(均为P<0.05);在第12、18周测试中,与对照组相比,中、高剂量组逃避潜伏期延长,而各染锰组的穿越平台次数均下降(均为P<0.05)。与对照组血浆锰含量[(13.16±5.45)μg/L]相比,低、中、高剂量染锰组的血浆锰含量均升高[分别为(55.84±11.62)、(82.21±8.26)、(115.58±21.31)μg/L,均为P<0.05);与对照组血浆BDNF含量[(232.64±75.37)ng/L]相比,中、高剂量染锰组的血浆BDNF含量均下降[分别为(145.80±46.14)、(93.21±44.92)ng/L,均为P<0.05]。血浆BDNF含量与穿越平台次数呈正相关,与逃避潜伏期及血浆锰含量呈负相关(均为P<0.05)。[结论]慢性锰染毒可致大鼠空间学习记忆能力下降,并降低血浆BDNF含量,血浆BDNF似可考虑作为锰染毒致大鼠学习记忆功能损害的效应生物标志。
[Objective] To observe the effects of different doses of chronic manganese exposure on learning and memory ability and its effect on the content of brain-derived neurotrophic factor (BDNF) in rats and to explore the effect of plasma BDNF content on the learning and memory of rats Feasibility of Biomarkers of Functional Impairment Effects. [Methods] Forty male SD rats were randomly divided into control group and low dose (5 mg / kg), medium dose (10 mg / kg) and high dose (20 mg / kg) Week 5 d, for 18 weeks. The learning and memory abilities of rats were detected by Morris water maze test at the 6th, 12th and 18th week respectively. At the end of the 18th week, the blood samples were collected and separated from plasma for 24 hours. The contents of manganese and BDNF in plasma were detected by graphite furnace atomic absorption spectrometry and ELISA kit respectively. [Results] During the 6th week of water maze test, compared with the control group, the high-dose group prolonged the escape latency and decreased the number of passing through the platform (all P <0.05); at the 12th and 18th week, compared with the control group Compared with the middle and high dose groups, the escape latency was prolonged, while the number of crossing platforms of each manganese group decreased (all P <0.05). Plasma manganese levels were significantly increased in the low, middle and high doses of Mn-d [(55.84 ± 11.62) and (82.21 ± 8.26), respectively, compared with those in the control group [(13.16 ± 5.45) μg / L] (115.58 ± 21.31) μg / L, all P <0.05). Compared with the control group, the levels of BDNF in the plasma were lower than those in the control group [(232.64 ± 75.37) ng / L] (145.80 ± 46.14) and (93.21 ± 44.92) ng / L, all P <0.05]. Plasma BDNF content and the number of cross-platform was positively correlated with escape latency and plasma manganese content was negatively correlated (P <0.05). [Conclusion] Chronic manganese exposure can decrease the spatial learning and memory ability of rats and decrease the content of BDNF in plasma. BDNF in plasma may be considered as an effective biomarker of impaired learning and memory function induced by manganese in rats.