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将初始体质量为(5.0±0.5)g的欧洲鳗鲡,经质量浓度为0.2mg/L孔雀石绿浸浴24h后换水1/2,再以0.1mg/L孔雀石绿浸浴24h,经2次换水,每次换水2/3,将污染鱼体捞出,移入无污染的水泥池,另以相同规格未经孔雀石绿浸浴的欧洲鳗鲡作为对照组,按《无公害欧洲鳗鲡静养池塘养殖技术规范》进行正常养殖管理。定期抽取试验组和对照组鳗鲡各10尾,测定规格后,采用高效液相色谱-串联质谱联用仪测定鳗鲡体内的孔雀石绿及无色孔雀石绿残留,孔雀石绿和无色孔雀石绿的方法检测限均为0.5μg/kg。结果表明,试验组鳗鲡养殖至240d,鱼体体质量达100g时,鳗鲡体内未检出孔雀石绿,而无色孔雀石绿残留量仍达(2.2±0.4)μg/kg;养殖至300d,鱼体体质量达182g时,无色孔雀石绿未检出;对照组试验过程均未检出孔雀石绿和无色孔雀石绿残留。孔雀石绿及无色孔雀石绿在欧洲鳗鲡体内代谢缓慢,且无色孔雀石绿在鳗鲡体内残留时间较孔雀石绿更长,种苗期间使用孔雀石绿,极易导致孔雀石绿在鳗鲡养成品中残留。
The European eel with the initial body mass of (5.0 ± 0.5) g was immersed in water for 1 h at a concentration of 0.2 mg / L malachite green and then immersed in 0.1 mg / L malachite green for 24 h. 2 changes of water, each changing water 2/3, remove the contaminated fish, moved to the non-polluting cement pool, the other without the same specifications malachite green European eel as a control group, according to “pollution-free Europe Eel 鲡 rearing pond culture technical specifications ”for normal breeding management. The experimental group and the control group were regularly sampled from 10 eels. After the specifications were determined, the malachite green and the colorless malachite green residue in the eel, the malachite green and the colorless malachite were determined by high performance liquid chromatography-tandem mass spectrometry Green method detection limit of 0.5μg / kg. The results showed that malachite green was not detected in the eel when the body weight of the fish reached 100g after culturing for 240 days, while the residual amount of malachite green remained at (2.2 ± 0.4) μg / kg. After cultured for 300 days, When the body mass of the fish reached 182g, the colorless malachite green was not detected; in the control group, neither malachite green nor the colorless malachite green residue was detected. Malachite green and colorless malachite green in the European eel body metabolism slowly, and the colorless malachite green in the eel body residue longer than malachite green, malachite green during seedling, can easily lead to malachite green in the eel Residues in finished products.