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为了解析蜻蜓凤梨(Aechmea fasciata)开花机理,本研究根据转录组中的片段信息,利用RACE技术从蜻蜓凤梨中获得一个光敏色素因子(PIFs)的c DNA全长序列,并将其命名为Af PIF4-1。Af PIF4-1基因c DNA全长1 343 bp,开放阅读框为1 296 bp,编码431个氨基酸。利用NCBI分析氨基酸序列结果显示:Af PIF4-1含有b HLH蛋白家族共有的功能结构域,与海枣(Phoenix dactylifera),大豆(Glycine max),玉米(Zea mays),番茄(Solanum lycopersicum)中的PIF同源性分别为52%、49%、42%、39%。进一步构建了35S::Af PIF4-1过表达载体,并获得转基因拟南芥植株,可为进一步探究凤梨科植物开花机理,进而调控市场应用前景奠定了基础。
In order to elucidate the mechanism of flowering of Aechmea fasciata, a full-length cDNA sequence of phytochrome C (PIFs) was obtained from the dragonfly pineapple by RACE technique based on the fragment information in the transcriptome and named Af PIF4 -1. The cDNA of Af PIF4-1 gene was 1 343 bp in length and 1 296 bp in open reading frame, encoding 431 amino acids. Analysis of the amino acid sequence using NCBI revealed that Af PIF4-1 contains functional domains shared by the bHLH family of proteins and is closely related to those found in Phoenix dactylifera, Glycine max, Zea mays, Solanum lycopersicum PIF homologies were 52%, 49%, 42%, 39% respectively. Further construction of 35S :: Af PIF4-1 overexpression vector and obtaining transgenic Arabidopsis plants may provide a basis for further exploring the flowering mechanism of bromeliads and further regulating the market application prospects.