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目的探讨胰岛素对新生大鼠缺氧缺血性脑损伤(HIBD)的保护作用。方法建立新生大鼠HIE模型,利用末端转移酶介导的原位缺口标记法,检测缺氧缺血后24h大脑皮质和海马的神经元凋亡情况。结果大脑皮质和海马部位小、中剂量胰岛素组凋亡细胞显著少于对照组;大剂量胰岛素组凋亡细胞数显著多于对照组。侧脑室小剂量胰岛素组血糖水平与对照组无显著差异;中、大剂量胰岛素组血糖水平显著低于对照组。结论适量的胰岛素对新生大鼠HIBD具有保护作用。
Objective To investigate the protective effect of insulin on hypoxic-ischemic brain damage (HIBD) in neonatal rats. Methods The neonatal rat model of HIE was established. The apoptosis of neurons in the cerebral cortex and hippocampus 24 hours after hypoxia-ischemia was detected by terminal transferase-mediated nick end labeling. Results The number of apoptotic cells in the small and medium dose insulin groups in the cerebral cortex and hippocampus was significantly less than that in the control group. The number of apoptotic cells in the high dose insulin group was significantly more than that in the control group. There was no significant difference in blood glucose level between the low dose insulin group and the control group. The blood glucose level of middle and high dose insulin group was significantly lower than that of the control group. Conclusion The appropriate amount of insulin has a protective effect on HIBD in neonatal rats.