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使用副睾及睾丸精子作胞浆内精子注射(ICSI),由于精于量少,并且绝大多数为非活动精子,鉴别精子的死活存在困难。在低渗肿胀试验中,水的流入使精子尾部卷曲,可以检验精子质膜的完整性。本文使用人精子注射田鼠卵模型,观察单个精子低渗肿胀试验方法可否用于ICSI中的精子选择。 用1ml纯水加入0.5ml羟乙基哌嗪乙烷磺酸(HEPES)缓冲Earle氏液,置备为单个精子环化介质(SSC),调渗透压为
The use of epididymal and testicular sperm for intracytoplasmic sperm injection (ICSI), due to the small amount of precision, and the vast majority of inactive sperm, identification of sperm death and survival difficulties. In the hypoosmotic swelling test, water influx crimps the tail of the sperm and tests the integrity of the sperm plasma membrane. In this paper, human sperm injection field mouse egg model to observe the single sperm hypoosmotic swelling test method can be used for sperm selection in ICSI. 0.5 ml HEPES-buffered Earle’s solution was added to 1 ml of pure water, and the solution was prepared into single sperm circular medium (SSC). The osmotic pressure was