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目的 为了探讨p16基因与食管癌发生、发展的关系。 方法 应用聚合酶链反应、单链构象多态分析(PCR-SSCP)方法检测30例食管癌中p16基因的纯合性缺失和突变情况,并分析p16基因变异与食管癌的病理关系。 结果 30例食管癌患者中4例为p16基因纯合性缺失,2例存在突变。6例阳性样品与其它样品在年龄、饮酒史方面差异有显著性(P<0.01),与淋巴结转移、病理分期有差异(P<0.05);而与性别、肿瘤长度、肿瘤部位、病理类型无差异(P>0.05)。 结论 抑癌基因p16在食管癌中存在基因变异,其在食管癌的发生、发展中起重要作用
Objective To explore the relationship between p16 gene and the occurrence and development of esophageal cancer. Methods The homozygous deletions and mutations of p16 gene in 30 esophageal carcinomas were detected by polymerase chain reaction and single strand conformation polymorphism (PCR-SSCP). The relationship between p16 gene mutation and esophageal cancer was analyzed. Results Among the 30 cases of esophageal cancer, 4 cases were homozygous deletion of p16 gene and 2 cases had mutation. There were significant differences between the 6 positive samples and other samples in the age and drinking history (P <0.01), but not with the lymph node metastasis and pathological stage (P <0.05), but not with gender, tumor length, tumor location, histological type Difference (P> 0.05). Conclusion The gene mutation of tumor suppressor gene p16 in esophageal cancer has an important role in the occurrence and development of esophageal cancer