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为提高物种鉴定的准确性,本研究采用DNA条形码技术对大亚湾生态监控区冬季采集的贝类样品进行了种类鉴定。结果表明,26个形态种中,有15个可以通过线粒体COI和16S r RNA基因的系统发育分析鉴定到种的水平。部分形态上难以鉴定的种类,如线缝摺塔螺(Ptychobela suturalis)和区系螺(Funa sp.)可以通过条形码实现有效鉴定。锯齿巴非蛤(Paphia gallus)、西格织纹螺(Nassarius siquijorensis)、爪哇拟塔螺(Turricula javana)等种类存在相当大的种内遗传距离,有存在隐存种的可能性。尽管基于线粒体COI和16S r RNA基因的种内遗传距离和属内种间的遗传距离发生重合,无明显的条形码间隙,但通过系统树的方法仍能有效鉴定物种。可见,DNA条形码技术能有效提高海洋贝类物种鉴定的准确性并发现隐存种。
In order to improve the accuracy of species identification, we used DNA barcode technology to identify the shellfish samples collected in winter in the Daya Bay Ecological Monitoring Area. The results showed that 15 out of 26 morphological species could be identified by phylogenetic analysis of mitochondrial COI and 16S rRNA genes. Some morphologically unidentifiable species, such as Ptychobela suturalis and Funa sp., Can be effectively identified by barcoding. Paphia gallus, Nassarius siquijorensis and Turricula javana have considerable intraspecies genetic distance and the possibility of existence of cryptosporidium. Although the intraspecific genetic distance based on the mitochondrial COI and 16S rRNA genes overlaps with the genetic distance between species in the genus, there is no significant gap between the barcodes, but the systematic tree approach can still identify the species effectively. Can be seen, DNA barcoding technology can effectively improve the identification of marine shellfish species and found that the hidden species.