论文部分内容阅读
采用在草间钻头蛛Hylyphantes graminicola的饮水和人工饲料中添加四环素的方法研究了蜘蛛体内沃尔巴克氏体(Wolbachia)的去除。同时,通过设置草间钻头蛛Wolbachia感染种群与未感染种群之间不同的交配方式,研究Wolbachia感染对草间钻头蛛生殖和适合度的影响。结果显示,在草间钻头蛛的饮水和人工饲料中添加四环素均能部分去除草间钻头蛛体内的Wolbachia,在供试最高浓度下,去除率分别为40%和50%。4种不同的交配组合下,草间钻头蛛雌蛛均能产卵,卵的孵化率差异不显著,Wolbachia感染对草间钻头蛛的生殖无影响。感染Wolbachia的草间钻头蛛所产第1卵袋的卵历期为6.65d,极显著高于未感染Wolbachia的卵历期(P=0.008)。感染与未感染Wolbachia的草间钻头蛛种群世代存活率、性比以及适合度均无显著差异。
The removal of Wolbachia in spiders was studied by adding tetracycline to Hylyphantes graminicola’s drinking water and artificial diet. At the same time, the effects of Wolbachia infection on the reproductive and fitness of bitter spiders were studied by setting different mating methods between Wolbachia infected and uninfected populations. The results showed that the addition of tetracycline to the water and feed of Artemisia australis could partially remove Wolbachia from the grasshopper, and the removal rates were 40% and 50% respectively at the highest tested concentration. Under 4 different mating combinations, the spider moths could spawn eggs with no significant difference in egg hatching rate. Wolbachia infection had no effect on the reproduction of grass moths. The ovum length of the first egg pocket produced by Wolbachia was 6.65 days, which was significantly higher than that of Wolbachia without infection (P = 0.008). There was no significant difference in survival, sex ratio and fitness between the infected and uninfected Wolbachia populations.