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目的:研究二至丸乙酸乙酯提取物(ethyl acetate extractsof Erzhi pill,EAEP)对体外肝细胞损伤的保护作用及其机理。方法:培养L-O2型肝细胞,采用CCl4和H2O2体外分别诱导肝细胞损伤,检测培养上清液中天门冬氨酸转换酶(AST)和丙氨酸氨基转换酶(ALT)水平,测定上清液中丙二醛(MDA)的含量和过氧化物岐化酶(SOD)活力及谷胱甘肽过氧化物酶(GSH-Px)活性,MTT法检测细胞存活和增殖活性。结果:①EAEP(0.32～40μg.mL-1)剂量组可明显降低由CCl4所致肝细胞培养上清液中AST和ALT水平及MDA含量的升高,还可提高由H2O2所致肝细胞存活率和SOD活力及GSH-Px活性的降低。②EAEP(0.32～40μg.mL-1)剂量组可使H2O2升高的肝细胞培养上清液中ALT和ALT水平及MDA含量明显降低或恢复,还可提高CCl4降低的肝细胞存活率和SOD活力及GSH-Px活性。结论:提示AEEP对体外肝细胞损伤有直接保护作用,该作用可能与其抗氧化作用有关。
Objective: To study the protective effect and mechanism of ethyl acetate extracts of Erzhi pill (EAEP) on hepatocellular injury in vitro. Methods: Liver cells were cultured in L-O2 medium. CCl4 and H2O2 were used to induce hepatocellular injury in vitro. The levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in culture supernatants were determined. The content of malondialdehyde (MDA), the activity of superoxide dismutase (SOD) and the activity of glutathione peroxidase (GSH-Px) in the supernatant of the supernatant were measured. The cell survival and proliferation were measured by MTT assay. Results: ①EAEP (0.32 ~ 40μg.mL-1) dose group can significantly reduce the CCl4-induced liver cell culture supernatant AST and ALT levels and MDA content increased, but also increase the H2O2-induced liver cell survival rate And SOD activity and GSH-Px activity decreased. ② EAEP (0.32 ~ 40μg.mL-1) dose group could significantly reduce the level of ALT and ALT and the content of MDA in liver cell culture supernatant with elevated H2O2, and also reduce the survival rate of liver cells and SOD activity of CCl4 And GSH-Px activity. Conclusion: It suggests that AEEP has a direct protective effect on hepatocellular injury in vitro, which may be related to its anti-oxidative effect.