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以氧化豆油水溶物为试验材料,在草鱼肠道黏膜细胞培养液中加入不同浓度氧化豆油水溶物,研究不同剂量氧化豆油水溶物在不同作用时间下对肠道黏膜细胞生长、细胞形态结构的影响。结果显示:添加111.06—888.48 g/L氧化豆油的水溶物作用6h显著降低细胞活性(P<0.05),细胞集落面积减小、细胞形态改变,其中在444.24 g/L氧化豆油的水溶物作用下培养细胞空泡变性,且脂肪滴沉积,线粒体肿胀。在222.12—888.48 g/L氧化豆油的水溶物作用下,3—9h内培养液中LDH活力显著增高(P<0.05);各时间点培养液中GSH-PX、SOD、T-AOC均有显著变化。结果表明,在培养液中添加111.06—888.48 g/L氧化豆油的水溶物作用12h内,对草鱼肠道黏膜细胞产生了损伤,表现为抑制细胞生长,改变细胞形态、结构,可能引起细胞膜脂质过氧化,导致膜结构破坏,且作用程度与添加浓度、作用时间相关。研究认为氧化豆油水溶物对草鱼肠道黏膜细胞具有显著性的损伤作用。
Oxidation of soybean oil water soluble material was used to study the effects of different doses of oxidized soybean oil aqueous solution on intestinal mucosal cell growth and cell morphology and structure in grass carp intestinal mucosal cell culture medium . The results showed that the water-soluble substance of 111.06-888.48 g / L oxidized soybean oil significantly decreased the cell viability (P <0.05), the cell colony area decreased and the cell morphology changed. Under the action of 444.24 g / L oxidized soybean oil Cultured cell vacuolar degeneration, and fat droplets deposition, mitochondria swelling. Under the action of aqueous solution of 222.12-888.48 g / L oxidized soybean oil, the activity of LDH in the culture medium was significantly increased (P <0.05) within 3-9h; GSH-PX, SOD and T-AOC in the culture medium at each time point were significantly Variety. The results showed that the intestinal mucosal cells of grass carp were damaged within 12 hours after adding 111.06-888.48 g / L aqueous solubles of oxidized soybean oil, which could inhibit the cell growth, change the morphology and structure of the cells, and may cause the cell membrane lipid Peroxidation, leading to the destruction of membrane structure, and the role of the degree and concentration, duration of action. The study found that oxidized soybean oil soluble substances on grass carp intestinal mucosa cells have a significant role in injury.