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以肝脏绿色荧光转基因斑马鱼T3(lfabp:EGFP)为模型,研究水环境典型重金属污染物铜(CuCl_2)、汞(HgCl_2)和铅(Pb(Ac)2)在较低浓度暴露下对仔鱼(72~144 hpf)肝脏发育的影响。结果显示,各暴露组存活率均在95%以上;CuCl_2和HgCl_2在1μmol/L时均能够引起鳔发育或充气异常;3种化合物均能够显著减小肝脏荧光面积和光密度。相同暴露浓度下,CuCl_2抑制肝脏发育的能力强于Pb(Ac)2和HgCl_2,但HgCl_2在0.01μmol/L时就能够显著抑制肝脏发育;CuCl_2和Pb(Ac)2抑制lfabp表达量的能力强于HgCl_2,且Pb(Ac)2在0.01μmol/L时就能够显著抑制lfabp表达。结果表明,3种重金属化合物均能够显著抑制肝脏发育;转基因斑马鱼仔鱼可以作为检测环境污染物肝脏发育毒性的首选模型。
(Lfabp: EGFP) was used as a model to study the effect of exposure to low concentration of copper (CuCl 2), mercury (HgCl 2) and lead (Ac 2) 72 ~ 144 hpf) liver development. The results showed that the survival rate of all exposure groups were above 95%; CuCl_2 and HgCl_2 at 1μmol / L were able to cause abnormal development or abnormal pneumatosis; three compounds were able to significantly reduce the liver fluorescence area and optical density. At the same exposure concentration, CuCl 2 inhibited liver development more strongly than Pb (Ac) 2 and HgCl 2, but HgCl 2 at 0.01 μmol / L significantly inhibited liver development; CuCl 2 and Pb (Ac) 2 had a strong ability to inhibit lfabp expression In HgCl_2, and Pb (Ac) 2 at 0.01μmol / L can significantly inhibit lfabp expression. The results showed that three kinds of heavy metal compounds can significantly inhibit liver development; transgenic zebrafish larvae can be used as a detection model of liver toxicity of environmental pollutants of choice.