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利用叶片切割湿度计可迅速取得水势测定值样本。不过,如果切面面积对样本体积的比率高,则相当小的叶样(0.24平方厘米)也可能导致蒸腾失水,从而造成测定误差。在生长室中用不同水分胁迫水平下的普通小麦植株所进行的试验表明:由平均7平方厘米的叶片(切面对体积的比率很低)测得的叶水势值接近于由0.24平方厘米切面得到的数值。方法间的一致性可能与湿度计腔内切面的迅速封闭有关(通长在切后3秒内封闭)。利用叶片切割湿度计测定了旗叶水势,并估算对照对胁迫条件下小麦的取样误差和试验误差。估算这些数值的目的在于确定为了获得小麦水势测定值的特定统计精确度所需要的样本数和重复数。
Water potential measurements can be quickly obtained using a vane cutting hygrometer. However, if the ratio of slice area to sample volume is high, a relatively small leaf sample (0.24 cm 2) may also cause transpiration dehydration, resulting in measurement errors. Experiments conducted on common wheat plants at different water stress levels in the growth chamber showed that the leaf water potential measured by an average of 7 cm <2> leaves (slice-to-volume ratio was very low) approached the cut-off of 0.24 cm < The value obtained. The consistency between the methods may be related to the rapid closure of the lumen in the hygrometer (the length of the seal is closed within 3 seconds after cutting). Leaf blade water potential was measured by blade cutting hygrometer and sampling error and experimental error of wheat under stress were estimated. The purpose of estimating these values is to determine the number of samples and the number of replicates required to obtain a specific statistical accuracy of the wheat water potential measurements.