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目的探讨微量元素锰和钼对亚硝酸钠(NaNO2)中毒小鼠存活时间的影响。方法将160只昆明种小鼠随机分为8个组,急性实验组中3组小鼠分别腹腔注射0.015mL/g生理盐水、0.01%氯化锰及0.1%钼酸钠,另一组腹腔注射0.015mL/g0.01%氯化锰和0.1%钼酸钠。毕后各小鼠立即分别腹腔注射5%的NaNO2,观察并记录每只小鼠存活时间。慢性实验4组小鼠分别每日腹腔注射0.25%NaNO20.015mL/g,进行染毒15d,其中3个组同时又分别腹腔注射0.015mL/g0.01%氯化锰、0.1%钼酸钠或同时腹腔注射0.015mL/g0.01%氯化锰和0.1%钼酸钠。第16天,各组小鼠分别腹腔注射0.015mL/g5%亚硝酸钠,观察并记录每只小鼠存活时间。结果急性实验及慢性实验中的实验组小鼠比对照组小鼠存活时间明显延长(P<0.01)。结论锰、钼对小鼠NaNO2中毒有抑制作用,且锰、钼同时补充比单一补充作用更强。
Objective To investigate the effects of trace elements manganese and molybdenum on the survival time of sodium nitrite poisoning mice. Methods 160 Kunming mice were randomly divided into 8 groups. In the acute experimental group, 3 mice were injected intraperitoneally with 0.015mL / g normal saline, 0.01% manganese chloride and 0.1% sodium molybdate respectively, 0.015 mL / g 0.01% manganese chloride and 0.1% sodium molybdate. After completion of each mouse immediately intraperitoneal injection of 5% NaNO2, observed and recorded each mouse survival time. Chronic experiment 4 mice were intraperitoneal injection of 0.25% NaNO20.015mL / g, for 15 days, three of which were simultaneously intraperitoneal injection of 0.015mL / g0.01% manganese chloride, 0.1% sodium molybdate or At the same time, intraperitoneal injection of 0.015mL / g 0.01% manganese chloride and 0.1% sodium molybdate. On the 16th day, mice in each group were injected intraperitoneally with 0.015mL / g of 5% sodium nitrite. The survival time of each mouse was observed and recorded. Results The survival time of experimental mice in experimental group and chronic experimental group was significantly longer than that in control group (P <0.01). Conclusion Manganese and molybdenum have inhibitory effects on NaNO2 poisoning in mice, and manganese and molybdenum supplementation are more effective than single supplementation.