我们观察了农吉利碱对HeLa细胞系形态学的影响。HeLa细胞培养于含有RPMI 1640、10％小牛血清及青、链霉素的培养液中(pH 7.2),并置37℃恒温培养箱培养。实验时将细胞接种于培养瓶内,同时置入培养瓶中1张盖片。在接种后的第3天,将其分为不加药的对照组和加药的实验组。实验组按加药的浓度不同,再分为每ml培养液含农吉利碱100μg、300μg、500μg 3组,每组各2瓶。于加药后的第3天,由培养瓶内取出盖片,经FAA We observed the effect of Nongliolide on the morphology of HeLa cell lines. HeLa cells were cultured in RPMI 1640, 10% fetal bovine serum and streptomycin (pH 7.2) and incubated in a 37 ° C incubator. Cells were seeded into culture flasks during the experiment and placed in flasks in flasks. On the 3rd day after inoculation, it was divided into the control group without drug addition and the dosing experimental group. Experimental group according to the concentration of different dosing, and then divided into per ml culture medium containing pendimethalin 100μg, 300μg, 500μg 3 groups, each 2 bottles. On the third day after dosing, the cover slip was taken out of the flask and passed through FAA