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目的 观察胃癌组织中cNOSmRNA表达分布情况 ,探讨其与胃癌侵袭转移和预后的关系。 方法 运用原位杂交技术 ,检测 119例原发性胃癌标本中cNOSmRNA的表达 ,并对所获得的 82份病例随访资料进行生存分析。 结果 胃癌组织中胃腺癌细胞、血管 淋巴管内皮细胞及巨噬细胞中均可见有cNOSmRNA表达 ;肿瘤细胞分化程度越低、恶性度越高 ,胃腺癌细胞和血管 淋巴管内皮细胞cNOSmRNA阳性表达率就越高 ;胃腺癌细胞和血管 淋巴管内皮细胞cNOSmRNA阳性表达率与胃癌侵袭深度、淋巴结转移以及TNM分期呈正相关 ;胃癌血管 淋巴管内皮细胞cNOSmRNA阳性表达者术后生存率较低 ;巨噬细胞中cNOSmRNA表达与胃癌组织学分型、侵袭深度、淋巴结转移、TNM分期及患者术后生存期无关。 结论 胃腺癌细胞和血管 淋巴管内皮细胞cNOSmRNA表达情况与肿瘤恶性程度、侵袭和转移潜能有关 ,血管 淋巴管内皮细胞上cNOSmRNA阳性表达提示患者预后不良
Objective To observe the distribution of cNOS mRNA in gastric carcinoma and to investigate its relationship with invasion, metastasis and prognosis of gastric carcinoma. Methods The in situ hybridization technique was used to detect the expression of cNOS mRNA in 119 cases of primary gastric cancer specimens. Survival data of 82 cases were obtained. Results The expression of cNOS mRNA in gastric adenocarcinoma cells, vascular lymphatic endothelial cells and macrophages was found in gastric cancer tissues. The lower the degree of tumor cell differentiation and the higher the malignant degree, the positive rate of cNOS mRNA in gastric adenocarcinoma cells and vascular lymphatic endothelial cells The positive rate of cNOSmRNA in gastric adenocarcinoma cells and vascular lymphatic endothelial cells was positively correlated with the invasiveness of gastric cancer, lymph node metastasis and TNM stage. The postoperative survival rate of cNOSmRNA positive expression in vascular lymphatic endothelial cells of gastric cancer was lower. In macrophages The expression of cNOS mRNA had no correlation with histological type, depth of invasion, lymph node metastasis, TNM stage and survival time of patients. Conclusions The expression of cNOSmRNA in gastric adenocarcinoma cells and vascular lymphatic endothelial cells is related to the malignancy, invasion and metastasis potential. The positive expression of cNOSmRNA on vascular lymphatic endothelial cells suggests that the prognosis is poor