水稻分蘖与植株碳氮化合物含量和分蘖相关基因表达量关系分析

来源 :东北农业大学学报 | 被引量 : 0次 | 上传用户:ww447978636
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以分蘖特性不同的4个寒地粳稻品种和2个籼稻品种为供试材料,通过盆栽试验研究水稻分蘖发生与植株可溶性糖、全氮含量及分蘖相关基因转录表达量关系。结果表明,分蘖发生起始时间、速度及最高茎数等分蘖特性主要受遗传因素控制,穗数型品种分蘖早生快发;在分蘖过程中高分蘖品种植株可溶性糖含量和糖氮比均显著高于低分蘖品种,全氮含量与此相反;分蘖过程中高分蘖品种OsTB1、OsD3和OsD14基因转录表达量均显著低于低分蘖品种,OsMADS57基因转录表达量均显著高于低分蘖品种;分蘖发生数与分蘖不同时期植株全氮含量呈不显著负相关,与可溶性糖含量、糖氮比及OsMADS57基因转录表达量呈显著正相关,与OsTB1、OsD3、OsD14基因转录表达量呈显著负相关。 Four cold-season japonica rice varieties and two indica rice varieties with different tillering characteristics were used as materials to study the relationship between tillering and soluble sugar content, total nitrogen content and tiller-related gene transcription in rice plants. The results showed that tillering initiation time, speed and the maximum number of stems and other tillering traits were mainly controlled by genetic factors, and spikelet type was faster in earliest and earlier tillering stage. Soluble sugar content and sugar-nitrogen ratio were significantly higher in tillering stage than in tillering stage The low tillering varieties and the total nitrogen content were the opposite. The transcript levels of OsTB1, OsD3 and OsD14 genes in tillering were significantly lower than those in low tillering varieties, and the transcript levels of OsMADS57 were significantly higher than those in low tillering tillers. There was no significant negative correlation between total nitrogen content of tillering plants and soluble sugar content, sugar / nitrogen ratio and the expression of OsMADS57 gene, but negatively correlated with the transcriptional expression of OsTB1, OsD3 and OsD14 genes.
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