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本文介绍一种实验室测定羊水胎粪比值的方法.用正常剖宫产穿刺取羊水和新生儿新鲜胎粪混和,制备6种不同浓度即15,10.0,7.5,5.0,3.0和1.5g 胎粪/100ml 羊水.分别吸入毛细玻璃管中,用血球比积仪离心,测出每一浓度的6个比值数.通过20例羊水和20例新生儿胎粪的测定,将羊水胎粪比值分为三级.比值小于4.5为Ⅰ级,胎粪浓度在5g/100ml 羊水以下,为胎粪轻度污染.比值在4.5～9.5之间为Ⅱ级,胎粪浓度为5g～10g/100ml 羊水,为胎粪中度污染.比值大于9.5为Ⅲ级,胎粪浓度在10g/100ml 羊水以上,为胎粪重度污染.对一未知胎粪污染的羊水用比积仪离心,根据比值数判定胎粪污染程度.此法简便、快速、经济,比目测更客观、准确.
This article describes a method for laboratory determination of amniotic fluid meconium ratio of normal cesarean section puncture taking amniotic fluid and newborn meconium were mixed to prepare six different concentrations of 15,10.0,7.5,5.0,3.0 and 1.5g meconium / 100ml of amniotic fluid were inhaled capillary glass tube with a hematocrit centrifuge to measure the concentration of each of the six ratio number.After 20 cases of amniotic fluid and 20 cases of neonatal meconium determination, the amniotic fluid meconium ratio were divided into Grade 3. The ratio of less than 4.5 for grade Ⅰ, meconium concentration of 5g / 100ml amniotic fluid below, mild meconium contamination for meningitis in the ratio of 4.5 to 9.5 for the grade Ⅱ, meconium concentration of 5g ~ 10g / 100ml amniotic fluid, as Meconium moderate pollution, the ratio is greater than 9.5 for grade Ⅲ, meconium concentration in 10g / 100ml amniotic fluid above, severe meconium contamination.An unknown meconium-stained amniotic fluid with a plotter centrifuge, according to the ratio of the number of meconium contamination This method is simple, fast, economical and more objective and accurate than visual inspection.