长期以来检测内毒素缺乏一种简单、快速而敏感的方法。1956年Bang发现给美洲鲎注入G细菌可引起致命的D1C。Lerin等1964年在鲎体内及试管内都证实微量内毒素就可以使鲎细胞溶解物产生凝胶。此后鲎的阿米巴样细胞溶解试验(Limulus amebocyte Lysate Test,以下简称LLT)遂被用来诊断临床疾病。常用的方法有试管凝胶法、玻片微量法、蛋白 A long way to detect endotoxin deficiency is a simple, fast and sensitive method. In 1956, Bang discovered that injecting G bacteria into the American shrimp causes deadly D1C. In 1964, Lerin et al. Demonstrated in vivo and in vitro tests that trace amounts of endotoxins produced gelatinous solutes. Subsequent Limulus amebocyte Lysate Test (LLT) was then used to diagnose clinical disease. Common methods are tube gel method, slide trace method, protein