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目的 探讨 Y染色体微缺失与原因不明性无精症、少精症所致男性不育的相关性 .方法 特发性少精症 18例、特发性严重少精症 12例、特发性无精症 5例及正常已生育健康男性 10例作为研究对象和正常对照 .应用多重 PCR技术 ,对精液基因组 DNA进行 Y染色体上连续的 18个序列标记位点检测 .结果 少精症 18例中发现 Y染色体微缺失 2例 ,严重少精症 12例中发现 Y染色体微缺失 3例 .无精症 5例和正常已生育男性 10例均末发现 Y染色体微缺失 .缺失形式 3种 .结论 Y染色体微缺失与精子发生障碍相关
Objective To investigate the relationship between Y chromosome microdeletions and unexplained azoospermia and oligozoospermia induced male infertility.Methods 18 cases of idiopathic oligozoospermia, 12 cases of idiopathic severe oligozoospermia, idiopathic 5 cases of spermatozoa and 10 cases of normal healthy male were used as research objects and normal controls.Multiple PCR was used to detect 18 consecutive sequence of semen genomic DNA on Y chromosome.Results In 18 cases of oligospermia Y chromosome microdeletion in 2 cases, severe oligozoospermia in 12 cases found Y chromosome microdeletion in 3 cases.Azoospermia in 5 cases and normal fertile men in 10 cases were found Y chromosome microdeletion.Defective form of 3. Conclusion Y chromosome Microdeletions are associated with spermatogenesis disorders