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目的:确定红果槲寄生[Viscum coloratum(Kom.)Nakai f.Rubroaurantiacum Kitag,VCR]的抗肿瘤有效部位。方法:以S180实体瘤小鼠作为动物模型,以抑瘤率为考察指标,对红果槲寄生75%乙醇提取物的不同溶剂萃取部位:石油醚萃取部分、氯仿萃取部分、乙酸乙酯萃取部分、正丁醇萃取部分以及水层部分分别进行了抗肿瘤活性筛选。将接种肿瘤的昆明小鼠在成瘤后按照瘤体大小随机分为7组,阳性对照组于ip环磷酰胺,给药剂量为100 mg·kg-1,其他试验组于同一时间ig给药,每天1次,给药剂量按生药量计为14.2 g·kg-1,空白对照组给予相同剂量的空白溶剂,连续给药8 d。结果:红果槲寄生提取物能明显抑制小鼠S180实体瘤生长,其中正丁醇萃取物组,平均抑瘤率为48.8%;氯仿萃取物组,平均抑瘤率为44.1%。结论:正丁醇和氯仿萃取部分抗肿瘤活性较强,为红果槲寄生抗肿瘤有效部位。
Objective: To determine the anti-tumor effective site of Viscum coloratum (Kom.) Nakai f. Rubroaurantiacum Kitag, VCR. METHODS: S180 solid tumor mice were used as animal models. The tumor inhibition rate was used as an index to examine the different solvent extraction fractions of the 75% ethanol extract from the red mistletoe: petroleum ether extraction, chloroform extraction, and ethyl acetate extraction. The extraction fraction of n-butanol and the aqueous fraction were screened for antitumor activity, respectively. The tumor-inoculated Kunming mice were randomly divided into 7 groups according to the tumor size. The positive control group was administered with ip cyclophosphamide at a dose of 100 mg·kg-1. The other groups were given ig at the same time. One time a day, the dose was calculated as crude drug amount of 14.2 g·kg-1. The blank control group was given the same dose of blank solvent for 8 days. RESULTS: The growth of S180 solid tumours was significantly inhibited by the extract of the red fruit mistletoe, of which the average inhibition rate was 48.8% in the n-butanol extract group and 44.1% in the chloroform extract group. Conclusion: Extracts of n-butanol and chloroform have strong antitumor activity, which is an effective anti-tumor site of red fruit mistletoe.