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为了解六溴环十二烷(HBCDs)在海洋沉积物中的好氧微生物降解特性,在以HBCDs为唯一碳源的无机培养基中添加海洋沉积物悬浮液,利用HPLC-MS/MS测定HBCDs降解率,并驯化分离得到HBCDs降解菌株,进行菌种鉴定.结果显示:海洋沉积物好氧微生物对HBCDs的降解半衰期为38-61 d;在120 d中HBCDs共降解了72%;从海洋沉积物中富集、分离得到5株能够以HBCDs为唯一碳源的降解菌株,分别命名为CTF711-A、CTF711-C、CTF711-D、CTF711-E和CTF711-X,结合形态学观察、生理生化试验和16S r DNA序列分析,将其依次鉴别为假单胞菌属、红球菌属、不动杆菌属、微杆菌属和盐单胞菌属,除假单胞菌属外,其余菌株对HBCDs的降解能力还尚未见报道.同时将这5株菌分别培养与混合培养,混合菌株对HBCDs降解率最高.本研究报道了5株不同属的HBCDs好氧降解菌株,且混合菌株对HBCDs降解效果较好,结果可为HBCDs的生物修复提供理论和应用依据.
To understand the aerobic microbial degradation of HBCDs in marine sediments, marine sediment suspensions were added to inorganic media containing HBCDs as the sole carbon source, and HBCDs were determined by HPLC-MS / MS Degradation rate, and domestication of HBCDs strains were isolated and identified strains.The results showed that: marine sediment aerobic microorganisms HBCDs degradation half-life of 38-61 d; in 120 d HBCDs total degradation of 72%; from the ocean sediments Five strains were identified as CTF711-A, CTF711-C, CTF711-D, CTF711-E and CTF711-X, respectively. According to morphological observation, physiological and biochemical Test and 16S r DNA sequence analysis, which were identified as Pseudomonas, Rhodococcus, Acinetobacter, Microbacterium and Halomonas genus, in addition to Pseudomonas, the other strains of HBCDs Of degradation ability has not yet been reported.At the same time these five strains were cultured and mixed culture, the highest degradation rate of mixed strains of HBCDs.This study reported five different genus of HBCDs aerobic degradation strains, and mixed strains of HBCDs degradation Preferably, the results provide a rationale for the biological repair of HBCDs Theory and application basis.