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选用朱砂叶螨Tetranychus cinnabarinus阿维菌素抗性品系和敏感品系,测定了热预刺激后其在极限高温下的存活率,并应用SDS-PAGE技术研究了热激蛋白(HSPs)的种类及其含量。结果表明:非致死的热预刺激能显著提高朱砂叶螨耐极限温度的能力。两个品系在不同温度热激处理后,其蛋白质种类和含量发生了变化。正常情况下,朱砂叶螨敏感品系与阿维菌素抗性品系相比缺失8条条带;敏感品系热激后,增加了分子量分别为97.2,74.3,62.4,53.0和30.3kDa的5条条带;抗性品系热激后没有特异蛋白带的产生,但进一步高温胁迫后有些蛋白表达增强。此结果有助于解释朱砂叶螨抗性品系存在高温适合度优势现象。
The Tetranychus cinnabarinus abamectin-resistant strain and susceptible strain of Tetranychus cinnabarinus were selected to determine their survival rate under extreme high temperature after thermal pre-stimulation. SDS-PAGE was used to study the types of heat shock proteins (HSPs) content. The results showed that non-lethal heat pre-stimulation can significantly increase the resistance to temperature-limiting temperature of Tetranychus cinnabarinus. After heat shock treatment at different temperatures, the two varieties changed their protein types and contents. Under normal conditions, there were 8 bands in the susceptible strain of T. cinnabarinus compared with the avermectin-resistant strain. After heat shock, 5 strains with the molecular weights of 97.2, 74.3, 62.4, 53.0 and 30.3 kDa There was no specific protein band after heat shock in the resistant strain, but some proteins were enhanced after further heat stress. This result is helpful to explain the high-temperature suitability of resistant strains of Tetranychus cinnabarinus.