NS1蛋白为流感病毒非结构蛋白,只在病毒侵入宿主细胞后产生。目前NS1蛋白对细胞整体水平上的作用仍不清楚,为了解NS1蛋白在病毒感染细胞中的作用,构建了重组质粒pCMV-myc-NS1并将其转染A549细胞,利用双向电泳技术检测了受NS1蛋白调控的宿主蛋白,以期从蛋白质组水平上研究禽流感病毒与宿主细胞间的相互作用。同时,还检测了转染NS1对细胞增殖和细胞周期的影响。结果显示,NS1在细胞中的表达,能够明显引起宿主细胞代谢的变化,并通过阻滞细胞周期的正常进行而减缓细胞的增殖。 The NS1 protein is a non-structural protein of influenza virus that is produced only after the virus has invaded the host cell. At present, the role of NS1 protein on the overall cell level remains unclear. To understand the role of NS1 protein in virus-infected cells, a recombinant plasmid pCMV-myc-NS1 was constructed and transfected into A549 cells. Two-dimensional electrophoresis NS1 protein regulatory host proteins in order to study the interaction between the avian influenza virus and host cells at the proteomic level. At the same time, the effect of transfected NS1 on cell proliferation and cell cycle was also examined. The results showed that the expression of NS1 in the cell can obviously cause the change of metabolism in the host cell and slow the cell proliferation by blocking the normal cell cycle.